Glucocorticoids enhance the potency of Schwann cell mitogens.

摘要: Previous studies have documented that cultured Schwann cells require serum-containing medium to respond maximally mitogens. We now report are able proliferate a mitogenic response in serum-free defined termed oligodendrocyte media (ODM). Glucocorticoids the essential component of ODM which allow cell proliferation medium. Charcoal treatment fetal calf serum decreases potency axolemma-enriched fraction (AEF) by 50%. The addition 2 microM hydrocortisone charcoal-treated restores 75% lost mitogenicity. These observations consistent with view glucocorticoids present potent co-mitogens for AEF-induced proliferation. synthetic glucocorticoid, dexamethasone, is more co-mitogen than hydrocortisone, maximal effect at concentrations less 10 nM. In contrast, other steroids including aldosterone, progesterone, testosterone, and 17 beta-estradiol no on enhancing AEF. glucocorticoid antagonists RU 486 dehydroepiandrosterone (DHEA), but not antiestrogenic compound tamoxifen, block results suggest glucocorticoid-induced mediated through receptor (GR) mechanism. detected immunoreactivity GR cytoplasm, nuclei grown lacking dexamethasone. 100 nM dexamethasone these cultures resulted nucleus. This data suggests working