A reliable technique for marking the location of extracellular recording sites using glass micropipettes

作者: Daniel J. Simons , Peter W. Land

DOI: 10.1016/0304-3940(87)90347-8

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摘要: A simple and highly reliable technique is described for marking the locations of extracellularly recorded neurons using double-barreled glass micropipettes. One barrel contains 3 M NaCl recording; other horseradish peroxidase (HRP) that iontophoretically ejected low currents. With appropriate processing tissue, small well-localized spots are produced contain a number HRP-filled neurons. The effective in sequential recording sites within individual electrode tracks multiple penetrations same microelectrode.

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