Pre-B Cell Leukemia Transcription Factor (PBX) Proteins Are Important Mediators for Retinoic Acid-dependent Endodermal and Neuronal Differentiation of Mouse Embryonal Carcinoma P19 Cells

摘要: Pre-B cell leukemia transcription factors (PBXs) act as cofactors in the transcriptional regulation mediated by Homeobox proteins during embryonic development and cellular differentition. PBX1 protein is expressed throughout murine development, its deletion mice disrupts chondrogenesis. PBX levels are also increased mouse embryonal carcinoma P19 cells retinoic acid (RA)-induced differentiation. To elucidate role of this process, we stably overexpressed PBX1b antisense mRNA (PBX1b-AS cells). PBX1b-AS did not differentiate to neuronal or endodermal following treatment with RA suggesting required for both processes. Furthermore demonstrated that regulate RA-dependent induction bone morphogenetic 4 (BMP4) Decorin (DCN) using small interfering RNA. Chromatin immunoprecipitation assays further directly bind promoter Bmp4 Dcn vivo a fashion. In addition, type I II BMP receptor were treatment; however, was PBX-independent. Taken together these data demonstrate RA-induced differentiation expression BMP4 DCN process.