[14] Ion microscopy in biology and medicine

摘要: Publisher Summary This chapter discusses the ion microscopy in biology and medicine. The microscope provides a powerful technique for studying subcellular distribution of elements biological systems under physiological, pathological, toxicological conditions. direct imaging with cell morphology lateral resolution -0.5/μm, an unambiguous mass spectrometric isotopic detection high sensitivity are important features research. hydrated matrix specimens requires careful sample fixation before analysis. Frozen-hydrated or frozen freeze-dried methods allow that preserves both chemical structural integrity living cell. With recent developments spatial many smaller cytoplasmic structures fall within range microscopy. Ion image quantification remains area future use stable isotopes prediction transport also challenging underexplored has reached stage where biomedical problems can currently benefit from its unique capabilities.