Subcellular imaging of calcium exchange in cultured cells with ion microscopy

作者: W. A. Ausserer , Subhash Chandra , G. H. Morrison

DOI: 10.1242/JCS.102.3.417

关键词:

摘要: Calcium to calcium exchange between intracellular and extracellular pools has been imaged directly in individual cells using stable 44Ca (98.78% enrichment) the nutrient medium an isotopic imaging technique, ion microscopy. Observations were made by mass 40 determine native (40Ca), 44 localize from that exchanged with cellular calcium. LLC-PK1 porcine kidney epithelial exposed 1.87 mM for 0, 1, 2, 5, 8, 20, 60 90 min, cryogenically prepared prior microscopic analysis. The cell nucleus, Golgi region remaining cytoplasm could be spatially resolved within about 0.5 microns microscope. On basis of kinetics 40Ca it was observed all three compartments had a rapidly exchanging pool calcium, which took 2 min exchange. A moderately identified 20 50% their total internal external less than min. classified as slowly pool. Isotopic images 39K 23Na recorded along assess health status cell. unique ability distinguish enters enormous potential studies transport under physiological pathological conditions.

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