Differential properties of two stably expressed brain-specific glycine transporters

摘要: Clonal cell lines stably expressing the glial glycine transporter 1b (GLYT1b) and neuronal 2 (GLYT2) from rat brain have been generated used comparatively to examine their kinetics, ion dependence, electrical properties. Differential sensitivity of transporters sarcosine is clearly exhibited by clonal lines. GLYT2 transports with higher apparent affinity than GLYT1b not inhibited any assayed compound, as deduced transport assays electrophysiological recordings. A sigmoidal Na+ dependence uptake stable observed, indicating involvement more one in process. cooperative behavior for suggested. One Cl- required cycles, although shows three times this GLYT2. The number expressed was sufficient allow recordings current two exhibits voltage both its glycine-evoked capacitive currents recorded absence substrate.