Real-time quantitative monitoring of hiPSC-based model of macular degeneration on Electric Cell-substrate Impedance Sensing microelectrodes

摘要: Age-related macular degeneration (AMD) is the leading cause of blindness in developed world. Humanized disease models are required to develop new therapies for currently incurable forms AMD. In this work, a tissue-on-a-chip approach was through combining human induced pluripotent stem cells, Electric Cell–substrate Impedance Sensing (ECIS) and reproducible electrical wounding assays model quantitatively study AMD. Retinal Pigment Epithelium (RPE) cells generated from patient with an inherited unaffected sibling were used test platform on which assay conducted RPE damage. First, robust real-time quantitative monitoring over 25-day period demonstrated establishment maturation layers microelectrode arrays. A spatially controlled layer damage that mimicked cell loss AMD then initiated. Post recovery, significant differences (P<0.01) migration rates found between case (8.6±0.46 μm/h) control lines (10.69±0.21 μm/h). Quantitative data analysis suggested achieved due lower cell–substrate adhesion line. The ECIS parameter (α) be 7.8±0.28 Ω1/2 cm line 6.5±0.15 Ω1/2 cm control. These findings confirmed using biochemical assays. model-on-a-chip powerful translational studies considerable potential investigate novel by enabling real-time, patient-specific repair studies.