Identifying Neisseria Species by Use of the 50S Ribosomal Protein L6 (rplF) Gene

摘要: The comparison of 16S rRNA gene sequences is widely used to differentiate bacteria; however, this can lack resolution among closely related but distinct members the same genus. This a problem in clinical situations those genera, such as Neisseria, where some species are associated with disease while others not. Here, we identified and validated an alternative genetic target common all Neisseria which be readily sequenced provide assay that rapidly accurately discriminates Ribosomal multilocus sequence typing (rMLST) using ribosomal protein genes has been shown unambiguously identify these bacteria. PubMLST database (http://pubmlst.org/neisseria/) was queried extract 53 from 44 genomes diverse species. Phylogenies reconstructed were examined, single 413-bp fragment 50S L6 (rplF) produced phylogeny congruent concatenated genes. Primers enabled amplification direct sequencing rplF designed validate vitro silico. Allele defined for fragment, particular names, stored on database, providing curated electronic resource. approach provides sequencing, replicated other organisms more required, it potential applications high-resolution metagenomic studies.