Preterm labor in the absence of acute histologic chorioamnionitis is characterized by cellular senescence of the chorioamniotic membranes

摘要: Background Decidual senescence has been considered a mechanism of disease for spontaneous preterm labor in the absence severe acute inflammation. Yet, signs cellular have also observed chorioamniotic membranes from women who underwent physiological process at term. Objective We aimed to investigate whether, histologic chorioamnionitis, or term exhibit markers senescence. Study Design Chorioamniotic membrane samples were collected Gestational age-matched nonlabor controls included. Senescence-associated genes/proteins determined using reverse transcription quantitative polymerase chain reaction (n = 7-9 each array; n = 26-28 validation), enzyme-linked immunosorbent assays each), immunoblotting 6-7 and immunohistochemistry 7-8 each). β-galactosidase activity 7-11 each) telomere length 15-22 evaluated. Results In without chorioamnionitis: (1) expression profile senescence-associated genes was different between groups (term labor) no labor), yet, there differences groups; (2) most differentially expressed among closely related tumor suppressor protein 53 pathway; (3) TP53 down-regulated compared their counterparts; (4) CDKN1A (gene coding p21) up-regulated (5) cyclin kinase CDK2 cyclins CCNA2 , CCNB1 CCNE1 group group; (6) concentration lower than (7) senescence-associated-β-galactosidase greater (8) phospho-S6 ribosomal reduced its counterpart but (9) significant relative study labor, labor). Conclusion are present those delivered labor. However, did not show consistent chorioamnionitis. These results suggest that pathways implicated pathological processes