Deformable liposomes for skin therapy with human epidermal growth factor: The effect of liposomal surface charge

摘要: Abstract The topical administration of exogenous human epidermal growth factor (hEGF) is a promising approach for improved chronic wound therapy. To develop therapeutically superior hEGF formulation, we prepared hEGF-containing neutral (NDLs), cationic (CDLs) and anionic (ADLs) deformable liposomes (DLs), respectively, since it expected that the liposomal surface charge can affect both physicochemical properties, their skin penetration potential therapeutic efficacy liposome-associated drug. All were similar size (300–350 nm) with high load (~80% entrapment efficacy). Among studied DLs, ADLs found to be most sustained release hEGF, as assessed in vitro using polyamide membrane. Ex vivo studies revealed all DLs excellent systems therapy no through full thickness was detected. provided depot exhibiting highest retention onto surface. also enhanced mitogenic activities fibroblasts compared NDLs CDLs after 48 hrs treatment. Moreover, significantly activity fibroblast solution (positive control). Similar trends observed keratinocytes 24 hrs We proved affects liposomes. nanosystem-based formulation localized wounds.