Differential antibody screening of cloned Plasmodium falciparum sequences expressed in Escherichia coli: procedure for isolation of defined antigens and analysis of human antisera.

摘要: Abstract We describe a procedure that uses polyspecific human sera for screening Escherichia coli colonies expressing cloned Plasmodium falciparum cDNA sequences in order to detect react differentially with different sera. This can be used two distinct purposes. First, it enables the isolation of clones encoding specified antigenic present complex mixture, without purification either antigens or antibodies by conventional procedures. requires antigen expressed E. and antisera are available differ substantially their reactivities component interest. To develop procedure, we shared many anti-P. specificities but differed only one was reactive isolate-specific S P. strain FCQ27/PNG (called FC27). Differential identified 30 clones, colony hybridization confirmed 25 these express S-antigen sequences. Second, identifies defined antibody within virtue ability any given clone. The has been here identify increase dramatically titer between acute convalescent phases malaria certain individuals and, hence, isolate corresponding antigens.