Hydrolysis of Esters of Monoribonucleotides

摘要: CONSIDERABLE attention has been paid in recent years to the hydrolysis of ribonucleic acids mononucleotides. It was shown1,2 that alkyl esters 2′- and 3′-monoribonucleotides (partial formula I ; R = alkyl) provided simple model substances which could be used for a more precise study mechanism hydrolysis. The overall process, effected by acid or alkali, results conversion (I) mixture (III) (IV). Both procedures involve unstable cyclic ester intermediate (II), is then hydrolysed isomeric mononucleotides (V). Since → (II) formally trans-esterification3, it seemed possible analogous process (V), is, migration phosphate residue without loss R, might also occur simultaneously.