Endocytotic Formation of Vesicles and Other Membranous Structures Induced by Ca2+ and Axolemmal Injury

摘要: Vesicles and/or other membranous structures that form after axolemmal damage have recently been shown to repair (seal) the axolemma of various nerve axons. To determine origin such structures, (1) we internally dialyzed isolated intact squid giant axons (GAs) and showed elevation intracellular Ca2+ >100 μm produced similar those in transected Ca2+-containing physiological saline; (2) exposed GA axoplasm salines observed did not removing glial sheath but severed GAs >99% their was removed by internal perfusion; (3) examined crayfish medial (MGAs) with time-lapse confocal fluorescence microscopy many injury-induced vesicles formed endocytosis axolemma; (4) cut ends MGAs electron most were single-walled at short (5–15 min) post-transection times, whereas more double- multi-walled probable longer (30–150 times; (5) differential interference contrast images large small lesions evoked injury responses which barriers dye diffusion amid an accumulation structures. These data suggest inflow or induces (including endocytotic vesicles) axonal form, accumulate, interact each other, preformed vesicles, damage.