Electrophoretic Techniques for the Detection of Human Microsatellite D19S884.
作者： Fazliana Mansor , Siti Sarah Hamzah , Liyana Zamri
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摘要: Background The detection and analysis of microsatellites is very important for the mapping genetic diseases because they are commonly used as markers. Microsatellite marker D19S884 has been associated with polycystic ovary syndrome (PCOS), most common reproductive endocrine disease women in their childbearing years. It responsible an estimated 70% cases anovulatory infertility. In this work, we detected DNA extracted from blood PCOS patients. Methods were amplified by polymerase chain reaction (PCR) using a pair specific primers tagged fluorescence to yield products 160-200 base pairs length. Alleles separated on 4% low-melting agarose gels; stained safe gel staining, GelRed™, which alternative ethidium bromide; visualised ultraviolet illumination. Results Bands observed, but base-pairs differences difficult distinguish. To identify each allele clearly, PCR also analysed capillary electrophoresis fragment where it was possible discriminate even case difference between two bases alleles. Conclusion article, present protocol that combines use identification biomarkers PCOS.
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