Deletions Generated by the Transposon Tn10 in the srl recA Region of the ESCHERICHIA COLI K-12 Chromosome

摘要: A negative regulatory gene for the srl operon (srlR) was recognized by characteristics of an insertion mutation generated transposon Tn10 determining tetracycline resistance. This finding is discussed in light previous hypotheses on regulation genes, which mediate metabolism glucitol (i.e., sorbitol). Mapping showed that order genes this region is: srlR srlD srlC recA alaS. Using two different methods, five mutations both and were detected. The phenotype conferred these mutations, UV sensitivity extreme recombination deficiency, characteristic standard point mutants. Three deletions also removed resistance nearby transposon. fourth ended at a distance from sufficient to allow separation following P1 transduction; our tests did not us conclude whether inversion or deletion. fifth deletion seemed end immediately adjacent boundary Tn10, proximal recA. Mechanisms generation are discussed.