Cellular biochemical assessment of steroid activity.

摘要: Publisher Summary This chapter presents the cellular biochemical assessment of steroid activity. Steroids can influence biochemistry in a number different ways. They may act by influencing gene activity, they inhibit enzymes direct action on enzyme molecule, or alter activity interference at co-enzyme substrate level as some types trans-hydrogenation mechanisms. have particular propensity for altering chemistry cells what amounts predominantly to their physical effects and subcellular membranes. Most these involve structural chemical phenomena. The effect steroids be studied conventional procedures. Disruptive analysis measures isolated enzyme, which very from its when attached natural matrix it is able interact with other systems. Willmer has reviewed earlier evidence concerning significance cell surfaces lipid monolayers. He drew attention fact that closeness packing phospholipid components depends relative proportion cholesterol phospholipid, strikingly altered changes component.