Electrophysiological functional recovery in a rat model of spinal cord hemisection injury following bone marrow-derived mesenchymal stem cell transplantation under hypothermia.
作者： Dong Wang , Jianjun Zhang
DOI: 10.3969/J.ISSN.1673-5374.2012.10.005
关键词:
摘要: Following successful establishment of a rat model spinal cord hemisection injury by resecting right tissues, bone marrow stem cells were transplanted into the lesions via caudal vein while maintaining rectal temperature at 34 ± 0.5°C for 6 hours (mild hypothermia). Hematoxylin-eosin staining showed that astrocytes gathered around site and formed scars 4 weeks post-transplantation. Compared with rats under normal temperature, hypothermia increased numbers proliferating (bromodeoxyuridine-positive cells), better recovery somatosensory-evoked motor-evoked potentials, greater Basso, Beattie, Bresnahan locomotor rating scores, an degree angle in incline plate test. These findings suggested combined mesenchymal transplantation effectively promoted electrical conduction nerve functional repair injury.
nrronline.org参考文章(35)
Janet E. Braisted, Susan M. Catalano, Robert Stimac, Timothy E. Kennedy, Marc Tessier-Lavigne, Carla J. Shatz, Dennis D. M. O'Leary, Netrin-1 Promotes Thalamic Axon Growth and Is Required for Proper Development of the Thalamocortical Projection The Journal of Neuroscience. ,vol. 20, pp. 5792- 5801 ,(2000) , 10.1523/JNEUROSCI.20-15-05792.2000
Florence R. Fischer, Jean D. Peduzzi, Functional recovery in rats with chronic spinal cord injuries after exposure to an enriched environment. Journal of Spinal Cord Medicine. ,vol. 30, pp. 147- 155 ,(2007) , 10.1080/10790268.2007.11753926
Arshak R. Alexanian, Wai-Meng Kwok, Danijel Pravdic, Dennis J. Maiman, Michael G. Fehlings, Survival of neurally induced mesenchymal cells may determine degree of motor recovery in injured spinal cord rats Restorative Neurology and Neuroscience. ,vol. 28, pp. 761- 767 ,(2010) , 10.3233/RNN-2010-0547
L Hu, ZG Zhang, YS Hung, KDK Luk, GD Iannetti, Y Hu, None, Single-trial detection of somatosensory evoked potentials by probabilistic independent component analysis and wavelet filtering. Clinical Neurophysiology. ,vol. 122, pp. 1429- 1439 ,(2011) , 10.1016/J.CLINPH.2010.12.052
Anna Sarnowska, Holger Braun, Steven Sauerzweig, Klaus G. Reymann, The neuroprotective effect of bone marrow stem cells is not dependent on direct cell contact with hypoxic injured tissue. Experimental Neurology. ,vol. 215, pp. 317- 327 ,(2009) , 10.1016/J.EXPNEUROL.2008.10.023
Patrick D. Lyden, Derk Krieger, Midori Yenari, W. Dalton Dietrich, Therapeutic hypothermia for acute stroke. International Journal of Stroke. ,vol. 1, pp. 9- 19 ,(2006) , 10.1111/J.1747-4949.2005.00011.X
Kirstin J. Beggs, Alex Lyubimov, Jade N. Borneman, Amelia Bartholomew, Annemarie Moseley, Robert Dodds, Michael P. Archambault, Alan K. Smith, Kevin R. Mcintosh, Immunologic consequences of multiple, high-dose administration of allogeneic mesenchymal stem cells to baboons. Cell Transplantation. ,vol. 15, pp. 711- 721 ,(2006) , 10.3727/000000006783981503
Karina T Wright, Wagih El Masri, Aheed Osman, Joy Chowdhury, William EB Johnson, None, Concise review:bone marrow for the treatment of spinal cord injury: mechanisms and clinical applications Stem Cells. ,vol. 29, pp. 169- 178 ,(2011) , 10.1002/STEM.570
De-Xiang Ban, Guang-Zhi Ning, Shi-Qing Feng, Ying Wang, Xian-Hu Zhou, Yang Liu, Jia-Tong Chen, Combination of activated Schwann cells with bone mesenchymal stem cells: the best cell strategy for repair after spinal cord injury in rats Regenerative Medicine. ,vol. 6, pp. 707- 720 ,(2011) , 10.2217/RME.11.32
Wen-Ge Liu, Zhen-Yu Wang, Zhu-Song Huang, Bone marrow-derived mesenchymal stem cells expressing the bFGF transgene promote axon regeneration and functional recovery after spinal cord injury in rats. Neurological Research. ,vol. 33, pp. 686- 694 ,(2011) , 10.1179/1743132810Y.0000000031