Phenotypic and genotypic characterization of human and nonhuman escherichia coli

摘要: Estuarine waters receive fecal pollution from a variety of sources, including humans and wildlife. Escherichia coli is one several coliform bacteria that inhabit the intestines many warm-blooded animals sometime contaminate water. Its presence does not specifically implicate human input, therefore it necessary to differentiate contamination sources accurately assess health risks. E. were isolated (HS) nonhuman (NHS) in Apalachicola National Research Reserve analyzed for fatty acid methyl ester (FAME), O-serogroup, pulsed-field gel electrophoresis (PFGE) profiles. For FAME PFGE analyses, there was no relationship between profile isolate source. Human source profiles less diverse than NHS isolates, conversely FAME. In contrast, O-serogrouping showed diversity HS vs. predominant O-serogroups differed significantly (P<0.01) those isolates.