CD101 is expressed by skin dendritic cells. Role in T-lymphocyte activation.

摘要: CD101 was first described in our laboratory using two different monoclonal antibodies, BA27 and BB27, recognizing a 140-kDa disulfide-bonded homodimeric polypeptide on small subset of circulating T lymphocytes most activated cells vitro. Further, it has been reported that intestinal mucosal expressed CD101. The gene coding for the antigen cloned found to be identical recently V7 antigen, corresponding type I trans-membrane protein with seven immunoglobulin-like loops its extracellular domain. To define surface proteins are involved skin dendritic cell (DC) localization or function, we looked expression CD1O1 DC migrating from human explants. majority these had phenotype Langerhans (LC)-like mature DC, i.e., HLA-DR+ CD1a+ CD1c+ CD11a+ CD11c+ CD40+ CD50+ CD54+ CD58+ CD80+ CD83+ CD86+. We by major LC-like DC. Next, studied effect anti-CD101 antibodies primary allogeneic soluble antigen-specific mixed DC-lymphocyte reactions. showed BB27 V7.1, inhibited T-lymphocyte proliferative responses inhibitory overcome high doses exogenous IL-2. As both molecules, determined achieved at responder T-cell level level. Thus, which is lymphocytes, also subpopulation This molecule plays role activation