Human insulin (Novo): chemistry and characteristics.

摘要: The amino acid sequence of human insulin was published in 1960. structure first confirmed 1982 by x-ray crystallography, which complete overlaps diffraction patterns were achieved on exposures from pancreas and prepared porcine insulin. Additional complementary identity tests like HPLC immunochemical cross-reactivity with anti-insulin sera have substantiated the Human (Novo) has been crude intertwining chromatographic purification processes for making monocomponent bovine two chemical reactions; a trypsin-catalyzed transpeptidation reaction nonenzymatic cleavage an ester bond. thus obtained complied purity specifications insulins. physico-chemical properties are similar to those insulin, hence analogous preparations therapy (Actrapid, Monotard, Protaphane) can be made. shares biologic characteristics other insulins, i.e., higher potency per milligram dry negligible immunogenicity rabbit test comparison conventional