Ladder-like amplification of the type I interferon gene cluster in the human osteosarcoma cell line MG63
作者： Narasimharao V. Marella , Michael J. Zeitz , Kishore S. Malyavantham , Artem Pliss , Sei-ichi Matsui
DOI: 10.1007/S10577-008-1267-X
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摘要: The organization of the type I interferon (IFN) gene cluster (9p21.3) was studied in a human osteosarcoma cell line (MG63). Array comparative genomic hybridization (aCGH) showed an amplification ∼6-fold which ended at both ends with deletion that extended throughout 9p21.3 band. Spectral karyotyping (SKY) combined fluorescence in-situ (FISH) identified arrangement ladder-like array 5–7 ‘bands’ spanning single chromosome termed ‘IFN chromosome’. Chromosome painting revealed IFN is derived from components chromosomes 4, 8 and 9. Labelling centromeric probes demonstrated 4 9 sequences co-localized each other similar banding pattern as well alternating clusters. In contrast, centromere not detected on chromosome. One amplified bands functional based its location constriction immunolocalization CENP-C protein. A model presented for generation involves breakage–fusion–bridge events.
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