Homozygous deletions within 9p21-p22 identify a small critical region of chromosomal loss in human malignant mesotheliomas

摘要: Previous DNA analyses have demonstrated that 9p13-p22 is a frequent site of chromosomal loss in leukemia, glioma, melanoma, and lung bladder carcinomas. Recent cytogenetic studies revealed recurrent alterations 9p malignant mesothelioma (MM). We performed gene dosage 23 MM cell lines, using probes for several 9p21-p22 loci (IFNB, IFNA/IFNW, D9S3, D9S126, D9S169, D9S171), to identify common region deletion. Homozygous and/or hemizygous deletions were identified 19 (83%) lines. losses (10 lines; 43%) occurred most often at the D9S171 IFNA/IFNW loci. In 8 2 or more examined found be homozygously lost; others displayed homozygous only locus. Results from our deletion mapping analysis suggest located between D9S126. The data presented here indicate allelic occurrence further delineate location putative tumor suppressor gene(s) D9S171. These lines may facilitate efforts define an even smaller critically deleted region, leading eventual cloning characterization this gene.