Mapping of the shortest region of overlap of deletions of the short arm of chromosome 9 associated with human neoplasia.

摘要: Deletions of the short arm chromosome 9 with a minimum region overlap at band 9p22 are frequently observed in acute lymphoblastic leukemia and gliomas. They also occur lower frequency lymphomas, melanomas, lung cancers, other solid tumors. These deletions often include entire interferon (IFN) gene cluster, which comprises about 26 interferon-alpha (IFNA), -omega (IFNW), and-beta-1 (IFNB1) genes, as well for enzyme methylthioadenosine phosphorylase (MTAP). By comparing microscopic genes lost molecular level, we have determined order these on 9p to be telomere-IFNB1-IFNA/IFNW cluster-MTAP-centromere. In few cell lines primary cells, that breakpoints within IFN cluster result partial loss genes. allowed us determine some or groups IFNA/IFNW cluster. Our current results map shortest various tumors between centromeric end MTAP locus.