Effect of extracellular adenosine 5'-triphosphate on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality, and in vitro fertilizing ability.

摘要: Intracellular adenosine 5'-triphosphate (ATP) is essential for supporting sperm function in the fertilization process. During cryopreservation, damage of mitochondrial membrane usually leads to compromised production intracellular ATP. Recently, extracellular ATP (ATPe) was introduced as a potent activator motility and fertilizing ability. This study aimed evaluate (1) levels frozen-thawed epididymal cat after incubation with ATPe (2) effects on parameters freezing thawing. Eighteen male cats were included. For each replicate, from two pooled one sample (N = 9). Each cryopreserved Tris-egg yolk extender into three straws. After thawing, first second straws incubated 0-, 1.0-, or 2.5-mM 10 minutes evaluated quality at minutes, 1, 3, 6 hours thawing The third straw concentration control treatment. Higher observed samples treated compared controls (0.339 ± 0.06 μg/2 × 10(6) vs. 0.002 0.003 sperm, P ≤ 0.05). In addition, promoted (56.7 5.0 53.3 4.4%, 0.05) progressive (3.1 0.2 2.8 0.4, 0.05), potential (36.4 5.5 28.7 4.8%, blastocyst rate (36.1 7.0 28.8 7.4%, controls. contrast, remarkably interfered acrosome integrity postthawed incubation. sum, present finding that optimal time postthaw under proper condition might constitute rationale studies other endangered wild felids regarding embryo development.