Treatment of sperm with extracellular adenosine 5’-triphosphate improves the in vitro fertility rate of inbred and genetically modified mice with low fertility

摘要: In vitro fertilization (IVF) is one of the most important techniques used for assisted reproduction in mouse colony management. As with natural mating, where mice have varying fertility indices, rates genetically modified (GM) [transgenic (Tg), knock out (KO) and congenic (Cg)] are influenced by their genetic background. Lines GM that poor a concomitant IVF outcome. Treatment sperm extracellular adenosine 5'-triphosphate (ATPe) enhanced outbred hybrid mice. The objective this study was to analyze effects using 5'-triphosphate-treated inbred wild type, lines, which standard did not work well. performed on C57BL/10SnJ, C57BL/6J, BALB/cJ NFS/N background strains type (WT) such as C57BL/6N, BALB/cAnN, B6129SF1 strains. Oocytes from superovulated females were fertilized same strain, either treated or ATPe. ATPe treatment outcome some WT strains, indicated percentage embryos progressed two-cell stage. There no marked difference between control groups development rate blastocysts culture, number pups born after transfer into recipient females. observed improvement results following transgenic KO potential solution improving rederivation gamete banking.