Collective cell migration over long time scales reveals distinct phenotypes.

摘要: Migratory phenotypes of metastasizing tumor cells include single and collective cell migration. While migration is generally less cooperative than that normal epithelial cells, our understanding precisely how they differ in long time behavior incomplete. We measure a model system cancer progression affects on scales determine perturbation cell–cell adhesions, specifically reduced E-cadherin expression, the phenotype. Time lapse imaging cellular sheets particle image velocimetry (PIV) are used to quantitatively study dynamics motion over ten hours. Long measured via finite Lyapunov exponents (FTLE) changes FTLE with time. find non-malignant MCF10A distinguished from malignant MCF10CA1a by both their short (minutes) (hours) dynamics. In addition, distinguish knockdown control, but increasing spatial correlations remain unchanged. Epithelial sheet includes cannot be captured metrics assess cooperativity based dynamics, such as instantaneous speed or directionality. The use incorporating data hours instead minutes allows us more describe E-cadherin, clinically relevant adhesion molecule, predict scale described here will robust predictive analysis velocity fields alone.