Malignant glioma-induced neuronal cell death in an organotypic glioma invasion model. Technical note.

摘要: Rapid growth and diffuse brain infiltration are hallmarks of malignant gliomas. The underlying molecular pathomechanisms these tumors, however, remain to be determined. authors present a novel glioma invasion model that allows researchers monitor consecutively tumor cell proliferation migration in an organotypic environment. Enhanced green fluorescent protein-labeled F98 rat cells were implanted into slice cultures obtained from hippocampus, was microscopically documented up 20 days vitro. Invasion along radially oriented migratory streams could observed 5 after implantation F98, human U87MG, mouse GL261 cells, whereas Be(2)c neuroblastoma HT22 hippocampal neurons failed invade the parenchyma. Following entorhinal cortex, death within infiltrated parenchyma as well neuroanatomically connected dentate gyrus. Application N-methyl-D-aspartate receptor antagonist MK801 culture medium significantly reduced neuronal degeneration gyrus, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate GYKI 52466 inhibited peritumoral cytotoxicity. This new address systematic manner pathways specific tumor-host interactions such necrosis.