[12] Mammalian membrane marker enzymes: Sensitive assay for 5′-nucleotidase and assay for mammalian 2′,3′-cyclic-nucleotide-3′-phosphohydrolase

摘要: Publisher Summary The assay described in this chapter is a sensitive, rapid, one-step reaction using long-lived isotope. Because tritium-labeled adenosine measured, the enzyme can be assayed presence of levels inorganic phosphate (as encountered some crude fractions) that preclude colorimetric determination enzymatically released phosphate. widely distributed and has been demonstrated large variety tissues including plant material, bacteria, snake venom, numerous mammalian tissues.