Ha-ras oncogene expression directed by a milk protein gene promoter: tissue specificity, hormonal regulation, and tumor induction in transgenic mice.

摘要: Abstract The activated human Ha-ras oncogene was subjected to the control of promoter region murine whey acidic protein (Wap) gene, which is expressed in mammary epithelial cells response lactogenic hormones. The Wap-ras gene stably introduced into mouse germ line five transgenic mice (one male and four females). expression observed glands lactating females two lines derived from female founders. tissue-directed hormone-dependent Wap conferred on oncogene. signals governing are located within 2.5 kilobases 5' flanking sequence. other founders did not express chimeric gene. In founder, integrated Y chromosome. Expression found salivary gland animals only. After a long latency, Wap-ras-expressing developed tumors. tumors arose tissues expressing Wap-ras--i.e., or glands. Compared corresponding nonmalignant tissues, enhanced