Differential effects of follicle-stimulating hormone, insulin, and insulin-like growth factor I on hexose uptake and lactate production by rat Sertoli cells.
作者： Ria B. Oonk , Ruud Jansen , J. Anton Grootegoed
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摘要: The stimulatory effects of follicle-stimulating hormone (FSH), insulin, and insulin-like growth factor I (IGF-I) on lactate production hexose uptake by Sertoli cells from immature rats were studied. time-courses the maximal FSH, IGF-I virtually identical. When incubated in presence FSH combination with insulin or (submaximal doses), additive but no pronounced synergistic observed. not dependent extracellular calcium. 2-Deoxy-D-glucose (2-DOG), an analogue D-glucose, was used to investigate transport system cells. Uptake 2-DOG linear time all intracellular phosphorylated up 30 min incubation; inhibited cytochalasin B, E. D-galactose, appeared be effective competitor uptake. Km influenced IGF-I. had effect Vmax uptake, whereas caused a 30% stimulation Vmax. It is concluded that stimulate cultured cells, only transport. may principally involve glycolytic enzyme activities.
sci-hub.se 参考文章(43)
J M Olefsky, Mechanisms of the ability of insulin to activate the glucose-transport system in rat adipocytes. Biochemical Journal. ,vol. 172, pp. 137- 145 ,(1978) , 10.1042/BJ1720137
J. Y. Cheung, J. M. Constantine, J. V. Bonventre, Cytosolic free calcium concentration and glucose transport in isolated cardiac myocytes. American Journal of Physiology-cell Physiology. ,vol. 252, ,(1987) , 10.1152/AJPCELL.1987.252.2.C163
Rolf F. Kletzien, James F. Perdue, The Inhibition of Sugar Transport in Chick Embryo Fibroblasts by Cytochalasin B Journal of Biological Chemistry. ,vol. 248, pp. 711- 719 ,(1973) , 10.1016/S0021-9258(19)44431-1
N. Toyoda, J.E. Flanagan, T. Kono, Reassessment of insulin effects on the Vmax and Km values of hexose transport in isolated rat epididymal adipocytes. Journal of Biological Chemistry. ,vol. 262, pp. 2737- 2745 ,(1987) , 10.1016/S0021-9258(18)61568-6
A Martz, B K Mookerjee, C Y Jung, Insulin and phorbol esters affect the maximum velocity rather than the half-saturation constant of 3-O-methylglucose transport in rat adipocytes. Journal of Biological Chemistry. ,vol. 261, pp. 13606- 13609 ,(1986) , 10.1016/S0021-9258(18)67063-2
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
N. H. P. M. Jutte, R. Jansen, J. A. Grootegoed, F. F. G. Rommerts, O. P. F. Clausen, H. J. van der Molen, Regulation of survival of rat pachytene spermatocytes by lactate supply from Sertoli cells Reproduction. ,vol. 65, pp. 431- 438 ,(1982) , 10.1530/JRF.0.0650431
Ria B. Oonk, J.Anton Grootegoed, Henk J. van der Molen, Comparison of the effects of insulin and follitropin on glucose metabolism by Sertoli cells from immature rats. Molecular and Cellular Endocrinology. ,vol. 42, pp. 39- 48 ,(1985) , 10.1016/0303-7207(85)90005-X
K. Suzuki, T. Kono, Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 2542- 2545 ,(1980) , 10.1073/PNAS.77.5.2542
John Cuppoletti, Eric Mayhew, Chan Y. Jung, Cytochalasin B binding to Ehrlich ascites tumor cells and its relationship to glucose carrier Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 642, pp. 392- 404 ,(1981) , 10.1016/0005-2736(81)90455-7