Expression and amplification of Her2, EGFR and cyclin D1 in breast cancer: immunohistochemistry and chromogenic in situ hybridization.

摘要: Determination of Her2, epidermal growth factor receptor (EGFR) and cyclin D1 status is now major clinical importance due to the development molecule-targeting drugs in anticancer therapy. Immunohistochemistry (IHC) chromogenic situ hybridization (CISH) are most simple convenient methods for evaluating gene alterations their protein consequences. The purpose present study was investigate EGFR on both IHC CISH 95 primary breast carcinomas. There substantial consistency between results Her2 EGFR, showing fair agreement overexpression amplification. However, D amplification not related overexpression. Moreover, there no correlation D1. were positively associated with histological grade, nuclear grade inversely correlated expression estrogen (ER) progesterone (PR). In ER-negative postmenopausal patients, strongly worse recurrence-free survival (P = 0.0087, P 0.0149, respectively). Overall, findings suggest that important predicting prognosis this should be evaluated carcinoma addition routine pathological practice.