Purification and Properties of Phosphoglucomutase from Potato Tubers
作者： RUSSELL PRESSEY
DOI: 10.1111/J.1365-2621.1967.TB09690.X
关键词:
摘要: SUMMARY— The level of phosphoglucomutase in potato tubers has been found to be about 400 times higher than previously reported. This enzyme purified 40-fold over the dialyzed crude extract by a procedure consisting fractionation with ethanol and acetone, ammonium sulfate, filtration through Sephadex G-100. is similar phosphoglucomutases obtained from other sources, but most closely resembles rabbit muscle enzyme. It requires Mg2+ cysteine for activity, activated preincubation cysteine, possesses molecular weight 63,000, exhibits optimal activity at pH 7.6, inhibited anions.
sci-hub.se 参考文章(14)
Ennis Layne, [73] Spectrophotometric and turbidimetric methods for measuring proteins Methods in Enzymology. ,vol. 3, pp. 447- 454 ,(1957) , 10.1016/S0076-6879(57)03413-8
Hermann Boser, Purification and analysis of phosphoglucomutase from potatoes Biological Chemistry. ,vol. 307, pp. 240- 246 ,(1957) , 10.1515/BCHM2.1957.307.1-2.240
J.G. JOSHI, T. HASHIMOTO, K. HANABUSA, H.W. DOUGHERTY, P. HANDLER, Comparative Aspects of the Structure and Function of Phosphoglucomutase Evolving Genes and Proteins#R##N#A Symposium Held at the Institute of Microbiology of Rutgers: the State University with Support from the National Science Foundation. pp. 207- 219 ,(1965) , 10.1016/B978-1-4832-2734-4.50022-X
William J. Ray, Gertrude A. Roscelli, A KINETIC STUDY OF THE PHOSPHOGLUCOMUTASE PATHWAY. Journal of Biological Chemistry. ,vol. 239, pp. 1228- 1236 ,(1964) , 10.1016/S0021-9258(18)91416-X
Norton Nelson, A PHOTOMETRIC ADAPTATION OF THE SOMOGYI METHOD FOR THE DETERMINATION OF GLUCOSE Journal of Biological Chemistry. ,vol. 153, pp. 375- 380 ,(1944) , 10.1016/S0021-9258(18)71980-7
Victor A. Najjar, The isolation and properties of phosphoglucomutase. Journal of Biological Chemistry. ,vol. 175, pp. 281- 290 ,(1948) , 10.1016/S0021-9258(18)57258-6
Jayant G. Joshi, Philip Handler, PHOSPHOGLUCOMUTASE. I. PURIFICATION AND PROPERTIES OF PHOSPHOGLUCOMUTASE FROM ESCHERICHIA COLI. Journal of Biological Chemistry. ,vol. 239, pp. 2741- 2751 ,(1964) , 10.1016/S0021-9258(18)93809-3
T. Ramasarma, J. Sri Ram, K.V. Giri, Phosphoglucomutase of green gram (Phaseolus radiatus). Archives of Biochemistry and Biophysics. ,vol. 53, pp. 167- 173 ,(1954) , 10.1016/0003-9861(54)90243-X
V. A. Najjar, M. E. Pullman, The occurrence of a group transfer involving enzyme (phosphoglucomutase) and substrate. Science. ,vol. 119, pp. 631- 634 ,(1954) , 10.1126/SCIENCE.119.3097.631
Sidney Harshman, John P. Robinson, Vincenzo Bocchini, Victor A. Najjar, ACTIVATION OF PHOSPHOGLUCOMUTASE. Biochemistry. ,vol. 4, pp. 396- 400 ,(1965) , 10.1021/BI00879A003