The use of a microcomputer to analyse differences between histograms derived from flow cytoflourimetric measurements

摘要: Abstract Computer programs which aid the interpretation of histograms fluorescence intensity obtained by flow cytofluorimetry are described. The methods employed have high reproducibility when used to estimate proportion cells contained in a subset population is distinguishable some degree enhancement intensity, e.g. lymphocyte peripheral blood mononuclear stained immunocytochemically with monoclonal antibody, or for detection those that undergone activation demonstrating an increase cellular RNA after stochiometric staining acridine orange. influence inter-observer variability and extraneous spikes caused cell culture debris has been investigated, guidelines set out use method. method can be efficiently relatively unskilled operators obtain higly reproducible results.