Differential Activation of Type I and Type II 3′, 5′-Cyclic Adenosine Monophosphate-Dependent Protein Kinases by Growth Hormone-Releasing Factor*
作者： Louise M. Bilezikjian , Jack Erlichman , Norman Fleischer , Wylie W. Vale
DOI: 10.1210/MEND-1-2-137
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摘要: Rat GH-releasing factor (rGRF) stimulated GH release and intracellular cAMP accumulation in cultured rat anterior pituitary cells with EC50 values of approximately 10 150 pm, respectively. Consistent an effect on cellular levels, rGRF the adenylate cyclase activity membranes value 60 pm. Using antisera directed against regulatory subunits type I II cAMP-dependent protein kinases, these enzymes were immunoprecipitated from cytosolic fraction order to monitor degree their activation by rGRF. Both isoenzymes rapidly activated incubated but different kinetics; full kinase was evident within 3-5 min occurred between 5 15 min. The magnitude differentially regulated a concentration-dependent manner. Somatostatin only partially attenuated rGRF-stimulated release, accumulation, activation. effective antagonizing at all concentrations intermediate significance this rGRF-induced differential two is discussed terms multiple effects somatotropic pituitary.
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