Immunoassays based on electrochemical detection using microelectrode arrays

摘要: Abstract We show that CombiMatrix’s VLSI arrays of individually addressable electrodes, using conventional CMOS integrated circuitry, can be used in detecting various analytes via immunoassay protocols. These microarrays provide over 1000 electrodes per square centimeter. The chips are coated with a porous material on which specific affinity tags synthesized proximate to selected electrode sites. CombiMatrix develop spatially multiplexed assay formats for biological entities wide range sizes, from small molecules cells. Antibodies tagged coded labels and then allowed self-assemble the appropriate Each analyte-specific antibody is chaperoned individual, predetermined locations by self-assembly process. resulting chip perform numerous different immunoassays, simultaneously. present new detection technologies based upon use active microelectrodes chip: redox enzyme amplified electrochemical detection. results human α1 acid glycoprotein, ricin, M13 phage, Bacillus globigii spores, fluorescein indicate this method one most sensitive available, limits attomole range. 4–5 logs analyte concentration, an volume 50 μl or less. system provides host immunoassays because large number available. how assays optimized maximum performance microarray platform.