Structure and expression of the human FHIT gene in normal and tumor cells.

摘要: The FHIT gene, encoded by 10 exons in a 1.1-kb transcript, encompasses approximately 1 Mb of genomic DNA, which includes the hereditary RCC t(3;8) translocation break at 3p14.2, FRA3B common fragile region, and homozygous deletions various cancer-derived cell lines. Because some these genetic landmarks (e.g., between untranslated 3 4, major region that viral integration site 4 5, cancer intron 5) do not necessarily affect coding yet apparently expression gene product, we examined locus its detail more than tumor-derived lines to clarify mechanisms underlying aberrant expression. We observed with continuous large deletions, included one or exons; discontinuous excluded exhibited heterozygous and/or Southern blot analysis for presence specific exons. Most alterations showed alteration transcripts absence diminution Fhit protein.