Three-Dimensional Rotating Wall Vessel-Derived Cell Culture Models for Studying Virus-Host Interactions.

摘要: The key to better understanding complex virus-host interactions is the utilization of robust three-dimensional (3D) human cell cultures that effectively recapitulate native tissue architecture and model microenvironment. A lack physiologically-relevant animal models for many viruses has limited elucidation factors influence viral pathogenesis host immune mechanisms. Conventional monolayer may support infection, but are unable form structures microenvironments mimic physiology and, therefore, limiting their translational utility. rotating wall vessel (RWV) bioreactor was designed by National Aeronautics Space Administration (NASA) microgravity later found more accurately reproduce features in vivo. Cells grown RWV bioreactors develop a low fluid-shear environment, which enables cells 3D tissue-like aggregates. wide variety tissues (from neuronal vaginal tissue) have been shown productive infection physiological meaningful responses. vivo-like characteristics cellular RWV-derived aggregates make them ideal systems pathophysiology responses necessary conduct rigorous basic science, preclinical studies.