作者: Masaaki Miyamoto , Takashi Fujita , Yoko Kimura , Mitsuo Maruyama , Hisashi Harada
DOI: 10.1016/S0092-8674(88)91307-4
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摘要: … Both DNAs were respectively labeled at the Hindlll site by [y-32p]ATP using T4 kinase. Briefly, 4 fmol of probe DNA (specific activity; 3000 cpm/fmol) was incubated in a 20 pI reaction …