Dermal fibroblast and epidermal keratinocyte functionality on titania nanotube arrays

摘要: Abstract Transcutaneous implants that penetrate through the depth of skin are used in numerous clinical applications, including prosthetics and dental implants. Favorable interactions between implant surface respective layers critical for long-term success transcutaneous implantable devices, hence, it is essential to understand physiologic response elicited by skin–biomaterial interactions. Recent studies have shown material surfaces provide topographic cues at nanoscale level may one possible solution enhanced biomaterial integration, thus preventing rejection. In this study titania nanotube arrays were fabricated using a simple anodization technique as potential interfaces devices. The vitro functionality human dermal fibroblasts epidermal keratinocytes evaluated on these (diameter 70–90 nm, length 1–1.5 μm). Cellular terms adhesion, proliferation, orientation, viability, cytoskeletal organization, differentiation morphology investigated up 4 days culture fluorescence microscope imaging, cell viability assay, indirect immunofluorescence scanning electron microscopy. results reported indicate increased fibroblast decreased keratinocyte proliferation arrays.