Sequence Variation in tcdA and tcdB of Clostridium difficile: ST37 with Truncated tcdA Is a Potential Epidemic Strain in China

摘要: ABSTRACT Clostridium difficile is a well-known nosocomial infectious pathogen. Research on C. infection has primarily focused strains such as the hypervirulent PCR ribotype 027 (sequence type 1 [ST1]) emerging in Europe and North America. However, other new ribotypes some countries have attracted attention, 17 (ST37) Asia Latin We collected 70 sequenced their toxin genes, tcdA tcdB . Multilocus sequence typing (MLST) was used to study population structure. In addition, and/or sequences of 25 isolates were obtained from GenBank. Single nucleotide polymorphisms (SNPs) identified analyzed. Phylogenetic analyses performed gene evolution. All divided into 16 types (denoted A01 -16 B01 -16, respectively). Hypervirulent strain RT027 A13B12, RT078 A14B10, whereas newly epidemic RT017 A15B13. SNP analysis suggests possibility recombination , perhaps through horizontal transfer. SNPs also found corresponding primers widely for detection. Our shows that ST037 shares few genotypic features its genes with known strains, indicating they fall unique clade. findings can be map relationships among more finely than done less sensitive methods, toxinotyping or even MLST, reveal inherent epidemiological characteristics.