Human Skin Collagenase: Localization and Distribution in Normal Human Skin*

摘要: ABSTRACT Collagenase activity was measured quantitatively from normal human skin cultured on radioactive, native, reconstituted collagen gels. A regional difference in enzyme demonstrated with the scalp having a specific greater than four-fold that of body skin. Isolated epidermis and dermis separately revealed major site collagenase production is upper or papillary portion no can be detected lower portions dermis. The shows only minimal collagenolytic which marked contrast to anuran tadpole where tailfin source collagenase. Freeze-thawing puromycin blocks appearance both whole isolated suggesting de novo synthesis occurs during time culture. Under certain circumstances, such as healing wound edge, capable actively synthesizing acts together underlying provide an specifically attacking molecule.