Beyond Complementation. Map-Based Cloning in Chlamydomonas reinhardtii

摘要: Chlamydomonas reinhardtii is an excellent model system for plant biologists because of its ease manipulation, facile genetics, and the ability to transform nuclear, chloroplast, mitochondrial genomes. Numerous forward genetics studies have been performed in Chlamydomonas, many cases elucidate regulation photosynthesis. One resultant challenges moving from mutant phenotype gene mutation causing that phenotype. To date, complementation has primary method cloning, but this impractical several situations, example, when complemented strain cannot be readily selected or case recessive suppressors restore New tools, including a molecular map consisting 506 markers 8X-draft nuclear genome sequence, are now available, making map-based cloning increasingly feasible. Here we discuss advances developed using strains mcd4 mcd5, which carry restoring photosynthesis chloroplast mutants. Tools not previously applied such as bulked segregant analysis marker duplexing, being implemented increase speed at one can go gene. In addition assessing applying current resources, outline anticipated future developments context newly extended initiative.