Comparative evaluation of the extraction and analysis of urinary phospholipids and lysophospholipids using MALDI-TOF/MS.

摘要: Abstract Lipids, particularly phospholipids (PLs) and lysophospholipids (LPLs), are attracting increasing scientific interest for their biological functions in cells potential as disease biomarkers Alzheimer’s several types of cancer. Urinary PLs LPLs could be ideal clinical biomarkers, because urine can collected easily noninvasively. However, due to very low concentrations compared with the relatively large quantity contaminants this matrix, efficient extraction sensitive detection required analyzing urinary LPLs. In study, various methods were optimized from a perspective. An lipid method matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) established using two external ionization standards an internal standard mix containing 13 human lipids. 9-Aminoacridine (9-AA) was useful effective MALDI-TOF/MS analysis all lipids both positive negative ion modes. it necessary determine proportional balance between extracted matrix. The efficiency reproducibility acidified Bligh Dyer excellent positively negatively charged Analysis small volumes most 9-AA MALDI at or below 5 mM. combined analytical procedures allowed rapid comprehensive screening samples.