Reactor optimization for α‐1,2 glucooligosaccharide synthesis by immobilized dextransucrase

摘要: The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts enzyme preparation drastically limit specific activity immobilized (4 U/mL alginate beads). Moreover, even absence diffusion limitation at batch conditions used, behavior is modified by entrapment so that yield increases α-1,2 glucooligosaccharides (GOS) are produced with a lower (46.6% instead 56.7%) have mean degree polymerization than free When catalyst used continuous reaction, reactor flow rate necessary to obtain conversion substrates very low, leading external resistance. As result, synthesis higher its accumulation within limits operational stability decreases glucooligosaccharide productivity. This effect can be limited using columns length diameter ratio ≥20, optimizing substrate concentrations feed solution: best productivity obtained was 3.74 g · U−1 h−1, an GOS 36%. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 276–284, 2001.