Functional investigation on aromatase in endometrial hyperplasia in polycystic ovary syndrome cases.
作者： Pan-Lin Zhao , Qiu-Fang Zhang , Li-Ying Yan , Shuo Huang , Yuan Chen
DOI: 10.7314/APJCP.2014.15.20.8975
关键词:
摘要: Objective: To explore the possible significance of aromatase P450 in endometrial hyperplasia with a background polycystic ovary syndrome (PCOS). Methods: Immunohistochemistry was used to determine expression endometrium PCOS patients. Semiquantitative analysis mRNA and protein level wasalso carried out by real-time quantitative RT-PCR method. After cells were stimulated testosterone letrozole vitro, estradiol (E 2 ) determined, cell assessed. Results: The increased endometria When cultured 10 -6 M testosterone, E culture medium increased. An inhibitory effect on generation observed when treated -5 letrozole. Conclusions: There is an patient endometrium. Androgen stimulation could enhance synthesis production vitro while do reverse.
koreascience.or.kr参考文章(25)
Serdar E. Bulun, Evan R. Simpson, Aromatase expression in women's cancers. Advances in Experimental Medicine and Biology. ,vol. 630, pp. 112- 132 ,(2008) , 10.1007/978-0-387-78818-0_8
Organization of the human aromatase p450 (CYP19) gene. Seminars in Reproductive Medicine. ,vol. 22, pp. 5- 9 ,(2004) , 10.1055/S-2004-823022
Kaoru Hirose, Kazuo Tajima, Miki Watanabe, Keitaro Matsuo, Satoyo Hosono, Hideo Tanaka, Toru Nakanishi, Hidemi Ito, Interaction between CYP19A1 polymorphisms and body mass index in the risk of endometrial cancer in postmenopausal Japanese women. Asian Pacific Journal of Cancer Prevention. ,vol. 12, pp. 2747- 2752 ,(2011)
H. Sasano, N. Harada, M. Ozaki, S. Sato, K. Watanabe, H. Niikura, A. Yajima, Aromatase in human endometrial carcinoma and hyperplasia. Immunohistochemical, in situ hybridization, and biochemical studies. American Journal of Pathology. ,vol. 146, pp. 491- 500 ,(1995)
Lili Liu, Lijun Wang, Jindan Zheng, Gang Tang, Leptin promotes human endometrial carcinoma cell proliferation by enhancing aromatase (P450arom) expression and estradiol formation European Journal of Obstetrics & Gynecology and Reproductive Biology. ,vol. 170, pp. 198- 201 ,(2013) , 10.1016/J.EJOGRB.2013.04.004
Maëliss Peigné, Didier Dewailly, Long term complications of polycystic ovary syndrome (PCOS) Annales D Endocrinologie. ,vol. 75, pp. 194- 199 ,(2014) , 10.1016/J.ANDO.2014.07.111
Kakali Sen, John C. Hackett, Coupled Electron Transfer and Proton Hopping in the Final Step of CYP19-Catalyzed Androgen Aromatization Biochemistry. ,vol. 51, pp. 3039- 3049 ,(2012) , 10.1021/BI300017P
Amudha Thangavelu, Matthew J. Hewitt, Naomi D. Quinton, Sean R. Duffy, Neoadjuvant treatment of endometrial cancer using anastrozole: A randomised pilot study Gynecologic Oncology. ,vol. 131, pp. 613- 618 ,(2013) , 10.1016/J.YGYNO.2013.09.023
Chao Gao, Yingmei Wang, Wenyan Tian, Yuanxi Zhu, Fengxia Xue, The therapeutic significance of aromatase inhibitors in endometrial carcinoma Gynecologic Oncology. ,vol. 134, pp. 190- 195 ,(2014) , 10.1016/J.YGYNO.2014.04.060
Hugo Maia Jr., Haddad, Casoy, Correlation between aromatase expression in the eutopic endometrium of symptomatic patients and the presence of endometriosis. International Journal of Women's Health. ,vol. 4, pp. 61- 65 ,(2012) , 10.2147/IJWH.S29154