Costimulation of T cell proliferation by a chimeric B7-2 antibody fusion protein specifically targeted to cells expressing the erbB2 proto-oncogene.

摘要: T cells require at least two signals for activation and clonal expansion. The first signal conferring specificity is initiated by interaction of the cell receptor with antigenic peptides in context MHC molecules. second, costimulatory can be provided surface molecules on APCs such as B7-1 (CD80) B7-2 (CD86), which interact their counter-receptors cells. absence presents one possible mechanism tumor to escape immune surveillance. In experimental models transfection B7 genes into result cell-dependent rejection. We have developed a novel approach direct molecule target Our based chimeric fusion protein that consists extracellular domain human fused single-chain Ab (scFv) specific ErbB2 protein, type I growth factor overexpressed high percentage adenocarcinomas. This B7-2(225)-scFv(FRP5) expressed yeast Pichia pastoris purified from culture supernatants functionally active binds ErbB2. localizes specifically ErbB2-expressing cells, thereby providing results enhanced proliferation syngeneic suggest effective vaccines cancer immunotherapy could created targeting ligands