A novel secreted splice variant of vascular endothelial cell growth inhibitor

摘要: SPECIFIC AIMSWe address the hypothesis that alternate forms of vascular endothelial cell growth inhibitor (VEGI) are expressed in cells and describe identification two novel isoforms VEGI. We demonstrate one isoforms, VEGI-251, is secreted its overexpression results inhibition attenuation human breast cancer xenograft tumors by interfering with tumor neovascularization.PRINCIPAL FINDINGS1. Detection multiple VEGI transcripts tissueThe initially discovered (accession number AF039390; referred to here as VEGI-174) had no effect on formed stable transfectants, unlike a recombinant form, sVEGI (see section 5, below). Overexpression full-length VEGI-174 did not give rise peptide cell-conditioned media. It thus plausible other exist nature whose activity may be mimicked sVEGI. Indeed, Northern blot anal...