Osteoblasts attachment on amorphous carbon films

摘要: In this work we studied the osteoblasts response to amorphous carbon (a-C) films deposited on stainless steel substrates with different surface textures. For cells, attachment substrate is first step in process of cell/surface interactions which affects subsequent cellular and tissue response. Amorphous are characterized by very smooth surfaces that imaged roughness many their applications rely property. However, biomedical field cell strongly influenced topography particularly, for cells it has been shown rough enhances differentiation. Therefore, modified order obtain values average roughness. The were abraded or fine-polished four roughness: 0.01, 1.5, 2.1 3.5 μm. Surface before after deposition a-C was evaluated profilometry scanning electron microscopy (SEM), while chemical composition determined X-ray photoelectron spectroscopy. Human used evaluate effect finishes adhesion. number attached a colorimetric technique 24 h incubation, morphological cytoskeletal changes monitored using SEM. increases monotonically attaining up 160% more than positive control.