Ross River virus envelope glycans contribute to type I interferon production in myeloid dendritic cells.

摘要: Alphaviruses are mosquito-transmitted viruses that cause significant human disease, and understanding how these pathogens successfully transition from the mosquito vector to vertebrate host is an important area of research. Previous studies demonstrated mammalian-cell-derived alphaviruses differentially induce type I interferons (alpha/beta interferon [IFN-α/β]) in myeloid dendritic cells (mDCs), where cell-derived virus a poor inducer IFN-α/β compared virus. Furthermore, reduced induction by attributed differential N-linked glycosylation (29). To further evaluate role viral envelope glycans regulating response, were performed assess whether actively inhibits or simply IFN-α/β. Coinfection using mammalian- Ross River (mam-RRV mos-RRV, respectively) indicated mos-RRV was unable suppress mam-RRV mDC cultures. Additionally, panel mutant lacking either individual multiple sites used demonstrate essential for high-level These results suggest failure due lack complex carbohydrates on virion rather than active suppression DC antiviral response.