Comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (W3A), parainfluenza virus type 3, and respiratory syncytial virus

摘要: Parainfluenza virus 5 (PIV5) infects a wide range of animals including dogs, pigs, cats, and humans; however, its association with disease in humans remains controversial. In contrast to parainfluenza 3 (PIV3) or respiratory syncytial (RSV), PIV5 is remarkably non-cytopathic monolayer cultures immortalized epithelial cells. To compare the cytopathology produced by these viruses relevant human tissue, we infected an vitro model ciliated airway epithelium measured outcomes cytopathology. PIV5, PIV3 and, RSV all cells, infection was dependent on sialic acid residues. Only PIV5-infected cells formed syncytia. resulted more rapid loss shedding into lumen. These studies revealed striking differences versus indicate extent determined cell-lines does not predict events differentiated